Publications

Submitted
SESSIONS INTERACTIVEPOSTER. 2005 IN VITRO BIOLOGY MEETING. Submitted.
De Silva MP, Senarath WW. A CORRELATION STUDY OF DENDROMETER BAND DATA AND PHENOLOGICAL EVENTS WITH TREE RING WIDTH MEASUREMENTS IN SOME TREES OF SRI LANKA. Submitted.
Pizon JRL, Nuñeza OM, Uy MM, Senarath WTPSK. GC-MS analysis and evaluation of in vitro antioxidant potential and total phenolics content of wild hops (Flemingia strobilifera (L.) WT Aiton). Submitted.
WTPSK S, Chandrasena K. GP. H.(1999). Micropropagation of Munronia pumila Wight (Binkohomba)-. Meliaceae Proceedings of the 55"" Annual Sessions, Sri Lanka Association for the Advancement of Science (SLAAS), Section D-227. Submitted.
Hewage S, Senarath WTPSK. Conservation of Zeuxine flava (Orchidacea) through tissue culture. Submitted.
2017
U SRMS, C KBMA, WTPSK S, C JG. In Vitro Propagation of Kaempferia Galanga (Zingiberaceae) and Comparison of Larvicidal Activity and Phytochemical Identities of Rhizomes of Tissue Cultured and Naturally Grown Plants. Journal of Applied Biotechnology & Bioengineering [Internet]. 2017;2(4). Publisher's VersionAbstract
Vegetative propagation of K. galanga Linn (Zingiberaceae) cannot fulfill the current demand for planting material and tissue culture offers an alternative means for mass propagation. Experiments were carried out on propagation of plants through direct organogenesis and comparison of larvicidal activity and phytochemicals present in rhizome of natural and tissue cultured plants in order to confirm the potential use of tissue cultured plants as an alternative to natural plants in commercial scale productions. In vitro shoot induction was optimized with rhizomee bud explants grown on MS medium supplemented with 2.0 mg/L Benzyl amino purine (BAP) and 0.5 mg/L IAA (Indole-3-acetic acid). MS medium supplement with 1.0 mg/L IAA and 0.2 mg/L Indol-3-butric acid (IBA) was identified as the best medium for root induction. Rooted plantlets were acclimatized successfully (100%) in a mixture of soil: sand: compost (1: 1: 1). Hexane found to be a better solvent for extraction of phytochemicals over methanol and the 50% hexane extract showed the highest larvicidal activity against the fourth instar larvae of A. aegypti. GC-MS analysis revealed the existence of the nine key compounds in both samples tested confirming the possibility of using tissue cultured plants as a substitute for natural plants in medicinal purposes.
2016
Buddhapriya AN, Senarath WTPSK. In Vitro Micropropagation of Gyrinops walla(GAERTH.) using Leaf Disc Explants. International Conference on Bioscience and Biotechnology [Internet]. 2016;1:11-14. Publisher's VersionAbstract
Gyrinops walla (Gaertn.) (Thymelaeaceae) is a slender tree which grows in wet zone regions in Sri Lanka. Potentiality of production of resinous natural product, agarwood, which used in perfumery and medicine manufacturing, grant a great demand for this species in worldwide. In vitro micropropagation technique may be a practicable solution to provide healthy planting materials in commercial scale, in order to overcome the problems caused to the natural population due to overexploitation. Thus, using leaf disc explant, possibilities of mass propagation through in vitro techniques was explored in in vitro study. Explants were surface sterilized using 0.2% carbendazim, 10% sodium hypochlorite (Clorox) and 70% ethanol each followed by two successive washings in sterile distilled water. Murashige and Skoog (MS) basal medium supplemented with varied concentrations of different plant growth regulators, 6-benzylaminopurine (BA and naphthaleneacetic acid (NAA) were tested for induction of calli from leaf discs. Out of different growth regulator combinations, rapid callus growth was observed in MS medium supplemented with 1.0 mg/L BA and 3.0 mg/L NAA within six weeks. Calli obtained were yellowish, compact and callus induction was mainly observed along the midrib. Calli obtain could either be used for mass propagation, through indirect organogenesis or for establishment of plant cell culture to obtain secondary metabolites. From the results obtain, it could be concluded that callus initiation is feasible through leaf disc explant. Keywords: Gyrinops walla, in vitro micropropagation, callus formation, plant growth regulators, leaf disc explant
Senarath WTPSK, Jayalath DT, Buddhapriya AN. Comparison of phytochemicals present in Aquilaria malaccensis Lam.(Agarwood) and Gyrinops walla Gaertn. Imperial Journal of Interdisciplinary Research [Internet]. 2016;2. Full Text
Gunasena MDKM, Senarath WTPSK. Comparison of phytochemicals present in locally available Sri Lankan and imported (Indian) fruits of Punica granatum (Lythracea). Imperial Journal of Interdisciplinary Research [Internet]. 2016;3. Full Text
D. Trono, OM Nuñeza MMUWTPSKS. In vitro alpha-amylase and antioxidant activities of bark extracts of charcoal tree (Trema orientalis Linn). International Journal of Biosciences [Internet]. 2016;8:33–46. Full Text
Odchimar NMO, Nuñeza OM, Uy MM, Senarath WTPSK. Antioxidant Activity, Total Phenolic Content, and GC-MS Analysis of the root of Kawilan (EmbeliaphilippinensisA. DC.). Bull. Env. Pharmacol. Life Sci [Internet]. 2016;5:42–47. Full Text
Pizon JRL, Nuñeza OM, Uy MM, Senarath WTPSK. Ethnobotany of Medicinal Plants Used by the Subanen Tribe of Lapuyan, Zamboanga del Sur. Bull. Env. Pharmacol. Life Sci [Internet]. 2016;5:53–67. Full Text
2013
Senarath WTPSK. COMPARATIVE GROWTH ANALYSIS OF TISSUE CULTURED CLONES OF CORSICAN PINE WITH NATURAL SEEDLINGS. Proceedings of International Forestry and Environment Symposium. 2013.
De Silva MAN, Senarath WTPSK. Development of a successful protocol for in vitro mass propagation of Celastrus paniculatus Willd.-a valuable medicinal plant. 2013.
Senarath WTPSK. EFFECT OF SOME SELECTED FUNGICIDES ON THE GROWTH OF TISSUES CULTURED IN VITRO. Proceedings of International Forestry and Environment Symposium. 2013.
Arachchi KAHKK, Senarath WTPSK. EFFECT OF THE PERIOD OF THE YEAR WHICH EXPLANTS WERE COLLECTED ON SHOOT GROWTH AND THE CALLUS FORMATION OF THE TISSUE CULTURED JACK FRUIT. Proceedings of International Forestry and Environment Symposium. 2013.

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