@proceedings {2239,
	title = {In Vitro Micropropagation of Gyrinops walla(GAERTH.) using Leaf Disc Explants},
	journal = {International Conference on Bioscience and Biotechnology},
	volume = {1},
	year = {2016},
	pages = {11-14},
	abstract = {Gyrinops walla (Gaertn.) (Thymelaeaceae) is a slender tree which grows in wet zone regions in Sri\&nbsp;Lanka. Potentiality of production of resinous natural product, agarwood, which used in perfumery\&nbsp;and medicine manufacturing, grant a great demand for this species in worldwide. In vitro\&nbsp;micropropagation technique may be a practicable solution to provide healthy planting materials in\&nbsp;commercial scale, in order to overcome the problems caused to the natural population due to
overexploitation. Thus, using leaf disc explant, possibilities of mass propagation through in vitro\&nbsp;techniques was explored in in vitro study. Explants were surface sterilized using 0.2\% carbendazim,\&nbsp;10\% sodium hypochlorite (Clorox) and 70\% ethanol each followed by two successive washings in\&nbsp;sterile distilled water. Murashige and Skoog (MS) basal medium supplemented with varied
concentrations of different plant growth regulators, 6-benzylaminopurine (BA and
naphthaleneacetic acid (NAA) were tested for induction of calli from leaf discs. Out of different\&nbsp;growth regulator combinations, rapid callus growth was observed in MS medium supplemented with\&nbsp;1.0 mg/L BA and 3.0 mg/L NAA within six weeks. Calli obtained were yellowish, compact and\&nbsp;callus induction was mainly observed along the midrib. Calli obtain could either be used for mass
propagation, through indirect organogenesis or for establishment of plant cell culture to obtain\&nbsp;secondary metabolites. From the results obtain, it could be concluded that callus initiation is feasible\&nbsp;through leaf disc explant.
Keywords: Gyrinops walla, in vitro micropropagation, callus formation, plant growth regulators,
leaf disc explant},
	url = {https://www.researchgate.net/publication/313729045_IN_VITRO_MICROPROPAGATION_OF_Gyrinops_walla_GAERTH_USING_LEAF_DISC_EXPLANTS},
	author = {Buddhapriya, AN and Senarath, WTPSK}
}
